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A phosphomimetic mutant of RelA /p65 at S er536 induces apoptosis and senescence: An implication for tumor‐suppressive role of S er536 phosphorylation
Author(s) -
Bu Yiwen,
Li Xiaoning,
He Yingchun,
Huang Chenfei,
Shen Yi,
Cao Yu,
Huang Dan,
Cai Chuan,
Wang Yuhong,
Wang Ziqi,
Liao DuanFang,
Cao Deliang
Publication year - 2015
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.29852
Subject(s) - phosphorylation , apoptosis , biology , cancer research , survivin , du145 , programmed cell death , lncap , senescence , caspase , microbiology and biotechnology , cancer cell , cancer , biochemistry , genetics
Hundreds of NF‐κB inhibitors have been developed for cancer therapy, but their clinical efficacy is unsatisfactory. Here we show that the phosphorylation activation at Ser536 of RelA/p65 protein, a main subunit in the NF‐κB family, may play a tumor‐suppressive role. In normal colon mucosa, RelA/p65 phosphorylation at Ser536 was increasingly increased with the maturation and apoptotic shedding of epithelial cells, but the phosphorylation at Ser536 was decreased in colon cancer. In colon (HCT116 p53 wt and p53 −/− ), breast (MCF7), and prostate (LNCaP and DU145) cancer cells, a phosphomimetic mutation of RelA/p65 at Ser536 (named p65/S536D) triggered dramatic apoptosis through affecting expression of a wide range of cell death/survival genes, such as Bim, Puma, Noxa, Bcl‐2 and survivin. In HCT116 cells, p65/S536D mutant upregulated Fas, insulted mitochondrial membrane potential, and triggered cleavage and activation of caspase‐3, 7, 8 and 9. A FasL neutralizing antibody (NOK1) prevented cell death induced by the p65/S536D. A pan inhibitor of caspases, Z‐VAD‐FMK (20 μM), blocked caspase‐mediated mitochondrial membrane depolarization. This p65/S536D also triggered senescence in HCT116 cells through a p16‐dependent pathway, but not in MFC7 due to lack of p16. Intratumoral delivery of the p65/S536D effectively suppressed tumor growth in nude mice. Together our data suggest that the phosphorylation of RelA/p65 at Ser536 may confer it a tumor‐suppressive role by inducing apoptosis and senescence, highlighting the importance of discriminating the function and active status of individual active sites in RelA/p65 when NF‐κB inhibitors are considered for targeted therapy of cancer.