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CD26 (dipeptidyl peptidase IV/DPP IV) as a novel molecular marker for differentiated thyroid carcinoma
Author(s) -
Tanaka Tetsuji,
Umeki Kazumi,
Yamamoto Ikuo,
Sakamoto Fujio,
Noguchi Shiro,
Ohtaki Sachiya
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910640508
Subject(s) - thyroid carcinoma , thyroid , dipeptidyl peptidase , pathology , staining , immunohistochemistry , pax8 , carcinoma , biology , thyroid cancer , endocrinology , medicine , enzyme , gene , biochemistry , transcription factor
In this report we show that CD26 (dipeptidyl peptidase IV/DPP IV) is a novel molecular marker for differentiated thyroid carcinoma. Northern‐blot analysis of 22 various thyroid tissues revealed that CD26 is a more specific marker of differentiated thyroid carcinoma than 3 proto‐oncogenes previously reported to increase mRNA expression in thyroid carcinomas: c‐met, c‐erb B‐2 and EGF‐R. A comparative study of 3 CD26 assays, Northern blotting, immunohistochemical staining and activity staining clearly showed that CD26 enzyme activity staining is the most specific assay for differentiated thyroid carcinoma, yet the easiest to perform. Activity staining of 216 thyroid tissues detected CD26 in all 52 papillary carcinomas and all 5 follicular carcinomas, while all 58 cases of Graves' disease were CD26‐negative. Among benign neoplasms, 54 of 55 adenomatous goiters and 29 of 33 follicular adenomas were CD26‐negative. Staining intensity of the enzyme activity was relative to the degree of CD26 mRNA expression. Southern‐blot study showed no gene amplification or major translocation of the CD26 gene in 7 papillary carcinomas examined. Based on this study, ectopic expression of CD26 in differentiated thyroid carcinomas is thought to be mainly caused by increased CD26 mRNA expression. In conclusion, CD26 activity staining is a simple, specific assay which should be added to the usual pathological examinations in order to distinguish differentiated thyroid carcinomas from benign thyroid diseases. © 1995 Wiley‐Liss, Inc.