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Neoplastic progression of human colorectal cancer is associated with overexpression of the stromelysin‐3 and BM‐40/SPARC genes
Author(s) -
Porte Henri,
Chastre Eric,
Prevot Sophie,
Nordlinger Bernard,
Empereur Sylvie,
Basset Paul,
Chambon Pierre,
Gespach Christian
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910640114
Subject(s) - stromal cell , metastasis , colorectal cancer , extracellular matrix , pathology , biology , cancer research , neoplastic transformation , cancer , medicine , carcinogenesis , microbiology and biotechnology
The interaction of neoplastic cells with the extracellular matrix is a critical event for the initiation of cancer invasion and metastasis. This study was designed to evaluate the potential implication of stromelysin‐3 (ST3), a newly identified member of the matrix‐degrading metalloproteinase family, and of BM‐40/SPARC, a glycoprotein associated with the extracellular matrix, during the progression of human colorectal cancers. We analyzed the relative abundance of ST3 and BM‐40/SPARC transcripts by Northern blot, and their distribution by in situ hybridization, in normal mucosa, benign adenomas, and primary colorectal adenocarcinomas and their liver metastases. The ST3 and BM‐40/SPARC transcripts were overexpressed in primary colorectal cancers and their liver metastases compared to non‐neoplastic mucosa. These transcripts were localized in stromal fibroblasts adjacent to the neoplastic foci. Overexpression of ST3 correlated with the progression of human colorectal tumors toward local invasion and liver metastasis. Induction of these genes also occurred in diverticulitis and digestive neoplasms such as gastric and esophageal carcinomas. © 1995 Wiley‐Liss, Inc.