Premium
Interphase cytogenetic analysis of erb B2 and topollα co‐amplification in invasive breast cancer and polysomy of chromosome 17 in ductal carcinoma in situ
Author(s) -
Murphy Dermot S.,
McHardy Peter,
Coutts Jacqueline,
Mallon Elizabeth A.,
David George W.,
Kaye Stanley B.,
Brown Robert,
Nicol Keith W.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910640106
Subject(s) - polysomy , breast cancer , ductal carcinoma , fluorescence in situ hybridization , biology , pathology , carcinoma in situ , chromosome 17 (human) , cancer , cytogenetics , gene duplication , interphase , cancer research , chromosome , genetics , medicine , gene
Abstract Breast cancer is a genetically complex disease. Fluorescence in situ hybridisation can be used to analyse the genetics of breast‐cancer progression by interphase cytogenetics. We have analysed the histological distribution of erb B2 and topollα co‐amplification in paraffin sections of invasive breast cancer and show that the co‐amplified loci share the same histological distribution in the tumour and have a similar nuclear distribution within individual nuclei. Regions of the tumours without amplification are easily recognized and tumours with erbB2 and topollα co‐amplification can be distinguished from those with erb B2 amplification alone. In addition, FISH was used to show polysomy of chromosome 17 in non‐invasive ductal carcinoma in situ of the breast and erb B2 amplification in both the invasive and non‐invasive components of a breast cancer biopsy. This report of an interphase cytogenetic analysis of non‐invasive breast carcinoma in situ demonstrates the usefulness of FISH for the genetic study of breast cancer progression. © 1995 Wiley‐Liss, Inc.