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High rate of MDR ‐1 and heterogeneous pattern of MRP expression without gene amplification in endometrial cancer
Author(s) -
Esteller Manel,
MartinezPalones Josep M.,
García Angel,
Xercavins Jordi,
Reventós Jaume
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910630609
Subject(s) - endometrial cancer , gene , biology , carcinoma , multiple drug resistance , endometrium , gene expression , gene duplication , polymerase chain reaction , cancer research , cancer , microbiology and biotechnology , pathology , andrology , medicine , drug resistance , genetics , endocrinology
The present study was undertaken to determine the pattern of expression of 2 molecular markers of multidrug resistance, the MDR ‐I and MRP genes, in endometrial carcinomas from 50 untreated patients using a PCR semi‐quantitative assay. In addition, PCR analysis of MDR ‐I and MRP gene amplification was obtained in each case. High rate of MDR ‐I expression without gene amplification was detected in all endometrial‐carcinoma samples, as well as in 10 adenomatous hyperplasias and 50 samples of normal endometrium. On the other hand, MRP appeared to be expressed at moderate levels in normal endometrial tissue, while relatively high levels of MRP expression without gene amplification were occasionally observed in endometrial‐carcinoma samples. © 1995 Wiley‐Liss, Inc .