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Cell kinetics of CD34‐positive hematopoietic cells following chemotherapy plus colony‐stimulating factors in advanced breast cancer
Author(s) -
Danova Marco,
Rosti Vittorio,
Mazzini Giuliano,
De Renzis Maria R.,
Locatelli Franco,
Cazzola Mario,
Riccardi Alberto,
Ascari Edoardo
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910630508
Subject(s) - haematopoiesis , cd34 , progenitor cell , bone marrow , medicine , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor , granulocyte , chemotherapy , cancer research , breast cancer , myeloid , stem cell , immunology , cancer , biology , cytokine , genetics
Bone‐marrow (BM) hematopoietic precursors are recruited into proliferative activity when colony‐stimulating factors (CSF) are sequenced with chemotherapy (CT). Previous studies suggested that further CT can be safely administered only when the increased proliferative activity of these cells has subsided, because most cytostatic drugs selectively damage cycling cells. The safest interval between CSF discontinuation and the start of the next CT course needs to be ascertained in vivo . Thirty patients with advanced breast cancer were treated with an intensified FEC regimen, planned at 21‐day intervals, sequenced with granulocyte‐macrophage (GM)‐CSF (15 patients) or granulocyte (G)‐CSF (15 patients). Using flow cytometry (FCM) we evaluated the proliferation kinetics of CD34 + BM hematopoietic progenitors before CT + CSF and at different times after CSF administration was stopped. FEC + GM‐ and FEC + G‐CSF sequences both induced a rapid and sustained increase in the percentage of BM myeloid precursors (BMMP%) and in the cycling status of CD34+ BM cells. However, while the BMMP% remained elevated in both cases after CSF were stopped, the enhanced proliferative activity of CD34+ cells decreased more rapidly after GM‐ than after G‐CSF. Using FCM, CD34 + BM‐derived hematopoietic presursor cell kinetics is readily evaluated in the clinical setting. The administration of CSF following CT increases both the proliferative activity of CD34+ BM cells and the BMMP%. After CSF were discontinued a kinetic refractoriness of hematopoietic progenitors was more evident after GM‐CSF than after G‐CSF. These data may be of value in designing clinical trials to avoid cytostatic damage to the BM hematopoietic stem‐cell compartment. © 1995 Wiley‐Liss, Inc .