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Effects of organic and inorganic selenium compounds on rat mammary tumor cells
Author(s) -
Ronai Ze'Ev,
Tillotson Joanne K.,
Traganos Frank,
Darzynkiewicz Z.,
Conaway C. Clifford,
Upadhyaya Parmod,
ElBayoumy Karam
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910630322
Subject(s) - selenium , mammary tumor , cell growth , cell cycle , dna , chemistry , fragmentation (computing) , cell , dna synthesis , cell culture , dna fragmentation , biochemistry , biology , apoptosis , programmed cell death , cancer , genetics , ecology , organic chemistry , breast cancer
To explore cellular effects of potent organoselenium chemo‐preventive agents we have used a rat mammary tumor cell line. We demonstrate that 1,4‐phenylenebis(methylene) selenocyanate ( p ‐XSC) at a dose of 5 μM is a more potent inhibitor of DNA, RNA and protein synthesis as well as of mitochondrial transmembrane potential than its chemopreventive counterparts benzyl selenocyanate (BSC) and sodium selenite. These differences were also reflected in reduced growth rate by 24 and 48 hr. Cell‐cycle and cell‐morphology analysis revealed that higher doses of p‐XSC (10 μM) caused DNA fragmentation which was accompanied with partial loss of nuclear stainability, whereas BSC caused a noticeable change in cell‐cycle distribution and extensive micronucleation. Overall, our results point to cellular targets of selenium compounds which may mediate their chemopreventive activities in mammary tissues.