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Cell‐cycle‐dependent invasion in vitro by rat ascites hepatoma cells
Author(s) -
Iwasaki Teruo,
Shinkai Kiyoko,
Mukai Mutsuko,
Yoshioka Kiyoko,
Fujii Yoshitaka,
Nakahara Kazuya,
Matsuda Hikaru,
Akedo Hitoshi
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910630223
Subject(s) - aphidicolin , cell cycle , biology , in vitro , gentamicin protection assay , cell , nocodazole , cell culture , metastasis , microbiology and biotechnology , cancer research , cancer , biochemistry , genetics , cytoskeleton
The relationship between cell cycle and experimental metastasis of tumor cells in vivo has been investigated, but it remains to be elucidated which step of metastasis, or whether tumor‐cell invasion in particular, depends on cell cycle. We previously reported an in vitro cell‐monolayer invasion (transcellular migration) assay system, in which the invasive capacity of tumor cells is measured by counting tumor cells penetrating beneath a cultured mesothelial cell monolayer after tumor‐cell seeding. Using our invasion assay system, the relationship between invasive capacity and cell‐cycle distribution of MMI cells, a highly invasive clone of rat ascites hepatoma AH130, was investigated. Invasive capacity of aphidicolin‐ or hydroxyurea‐synchronized tumor cells enriched in G 1 /S—early S‐phase cells was about 2 to 6 times higher than that of asynchronous cells. According to time‐course experiments to examine the relationship between invasive capacity and the size of fraction of cells in each phase after release from an aphidicolin or a nocodazole block, it was suggested that MMI cells are most invasive in G 1 /S‐S phase. Phagokinetic assay using colloidal gold particles showed that one possible reason for the enhanced invasiveness might be the increased cell motility in such phases, as suggested by the in vitro invasion assay.