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EGFR gene amplification ‐ rearrangement in human glioblastomas
Author(s) -
Schwechheimer Karl,
Huang Su,
Cavenee Webster K.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910620206
Subject(s) - immunostaining , biology , exon , microbiology and biotechnology , epidermal growth factor receptor , gene duplication , fusion gene , polyclonal antibodies , complementary dna , gene rearrangement , gene , southern blot , extracellular , blot , immunohistochemistry , cancer research , antibody , receptor , genetics , immunology
Immunostaining using an affinity‐purified rabbit polyclonal antibody against the extracellular domain of the epidermal‐growth‐factor receptor (EGFR) showed over‐expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern‐blotting technique using a full‐length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over‐expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion‐rearrangement of 801 base pairs between exons 2 to 7, resulting in an in‐frame fusion of exons I and 8. © 1995 Wiley‐Liss, Inc.