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Human and viral interleukin‐10 in Hodgkin's disease, and its influence on CD4 + and CD8 + T lymphocytes
Author(s) -
Ohshima Koichi,
Suzumiya Junji,
Akamatu Minoru,
Takeshjta Morishige,
Kikuchi Masahiro
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910620103
Subject(s) - immunostaining , epstein–barr virus , cd8 , in situ hybridization , biology , virus , antigen , cytokine , microbiology and biotechnology , virology , rna , interleukin 4 , immunology , gene , gene expression , immunohistochemistry , biochemistry
The Epstein‐Barr virus (EBV) is closely related to Hodgkin's disease (HD), while the BCRF‐I (viral [v] IL‐10) gene of the EBV is highly homologous to the human interleukin‐10 (h IL‐10) gene. To investigate the relationship of IL‐10 and HD, we performed both immunostaining and in situ hybridization (ISH) in 30 cases of HD. The presence of EBV in Hodgkin (H) and Reed‐Sternberg (RS) cells was seen in 16 of the 30 cases, by ISH of the EBV EBER‐I region and/or immunostaining of latent membrane protein (LMP‐I). Of the 16 EBV‐positive cases, 12 also showed IL‐10 antigen (Ag) in H and RS cells by immunostaining, 5 of the 16 demonstrated hIL‐10 RNA by ISH and 14 of the 16 showed vIL‐10 RNA. But only 2 of the 14 EBV‐negative cases showed IL‐10 Ag, and one of them showed hIL‐10 RNA, while none demonstrated vIL‐10 RNA. The T cells in the HD‐involved tissues were found to be mainly CD4‐positive T cells, and had no association with EBV infection. However, the lymphocytes surrounding H and RS cells were more frequently CD4 cells and rarely CD8 cells in the EBV‐positive cases, in contrast with the EBV‐negative cases. The above results indicate that an EBV infection influenced both cytokine synthesis and the response of T cells in HD. © 1995 Wiley‐Liss Inc.