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p53 mutations in esophageal tumors from high‐incidence areas of China
Author(s) -
Liang YuanYuan,
Estève Asuncion,
MartelPlanche Ghyslaine,
Takahashi Satoru,
Lu ShihHsin,
Montesano Ruggero,
Hollstein Monica
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910610505
Subject(s) - incidence (geometry) , medicine , china , mutation , cancer research , oncology , pathology , gastroenterology , biology , genetics , gene , geography , physics , archaeology , optics
Carcinomas of the upper digestive tract (squamous‐cell carcinoma of the esophagus, adenocarcinoma of the cardia) from 24 patients residing in Linxian (China) and near‐by high‐incidence areas were analyzed for mutations in exons 5–8 of the p53 tumor‐suppressor gene. Mutations were identified by polymerase chain reaction amplification and direct sequencing in 50% of the specimens. Eleven tumors harbored a single base‐pair substitution leading to either an amino‐acid substitution (8 tumors) or a chain‐termination signal (3 tumors), and one tumor revealed a 15‐bp deletion in exon 7 with a silent base substitution adjacent to the deletion site. Mutations occurred in all 4 exons examined, with a preponderance in exon 5. Of the 6 mutations identified among the 14 adenocarcinomas examined, 3 were G to T transversions, a mutation that has thus far been absent from reported mutations in Barrett's esophageal adenocarcinomas and dysplasias from patients residing in Europe and North America. © 1995 Wiley‐Liss, Inc .