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α v ‐Integrins in human melanoma: Gain of α v β 3 and loss OF α v β 5 are related to tumor progression in situ but not to metastatic capacity of cell lines in nude mice
Author(s) -
Danen Erik H. J.,
Jansen Kees F. J.,
Van Kraats Annemieke A.,
Cornelissen Ine M. H. A.,
Ruiter Dirk J.,
Van Muijen Goos N. P.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910610411
Subject(s) - melanoma , immunohistochemistry , pathology , tumor progression , metastasis , cell culture , primary tumor , monoclonal antibody , integrin , cancer research , staining , medicine , cancer , biology , cell , antibody , immunology , genetics
We investigated the expression of α v ‐integrins in different stages of human cutaneous melanocytic tumor progression. We observed that α v β 5 was the α v ‐integrin expressed in all common nevocellular nevi, in 78% of dysplastic nevi, in 63% of early primary melanomas, in 43% of advanced primary melanomas, and in 33% of melanoma metastases. Hence, loss of α v β 5 expression was related to melanocytic tumor progression. In line with earlier reports, α v β 3 was exclusively detected in advanced primary melanomas and metastases (24% and 50% respectively). Staining with anti‐α v monoclonal antibodies (MAbs) in lesions where both α v β 3 and α v β 5 were absent showed that alternative α v ‐integrins were expressed in advanced primary melanomas and metastases. By FACS analysis, we determined expression of α v β 5 and α v β 3 in 4 human melanoma cell lines with different metastatic capacities after s.c. inoculation into nude mice. One of the non‐metastatic and both highly metastatic cell lines expressed α v β 5 at their surface. Surprisingly, α v β 3 was detected exclusively in the non‐metastatic cell lines. Absence of α v β 3 in the highly metastatic cell lines was confirmed by lack of immunoprecipitation from 35 S‐methionine‐labeled cells and by absence of immunohistochemical staining on primary and metastatic xenograft lesions. Our findings indicate that α v β 5 expression is often lost in advanced stages of melanocytic tumor progression in situ , while α v β 3 is acquired, but that a decrease in α v β 5 and an increase in α v β 3 expression are not necessarily related to the metastatic behavior of human melanoma cells in nude mice. © 1995 Wiley‐Liss, Inc .