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CDK1 is a marker of proliferation in human lymphoid cells
Author(s) -
Włowiec Dariusz,
Deviller Philippe,
Simonin Denis,
Souchier Catherine,
Rimokh Ruth,
Benchaib Mehdi,
Bryon PaulAndré,
Ffrench Martine
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910610318
Subject(s) - cyclin dependent kinase 1 , biology , cell cycle , messenger rna , flow cytometry , microbiology and biotechnology , kinase , cell growth , cell , biochemistry , gene
To better understand the relationship between the proliferation of human lymphoid cells and the expression of cdk1, a catalytic subunit of the histone HI kinase (HIK), we examined its mRNA and protein content in 3 B‐cell lines: Ramos, Reh‐6 and IARC 963. Cells were elutriated according to their position in the cell cycle. Cell fractions were analyzed for cdk1 mRNA and protein cellular content by Northern blot and immunoblot, respectively, as well as for H1K activity. Both mRNA and protein amounts and H1K activity varied according to cell cycle phase, the lowest values being observed in G 1 ‐enriched fractions. For comparison, elutriated fractions were also tested for the expression of cdk2 and cdk4 proteins. Both showed some variations among fractions, but they were less clear than those of cdk1. We also tested 29 samples of lymphoid neoplastic and non‐neoplastic tissues for proliferative activity (percentage of S and G 2 /M cells estimated by flow cytometry) and expression of cdk1, cdk2 and cdk4 proteins. We found a significant correlation between the percentage of cells in S or S + G 2 /M phases and cdk1 protein content but not cdk2 or cdk4 content. We conclude that cdk1 expression in human lymphoid cells varies during the cell cycle at both mRNA and protein levels. © 1995 Wiley‐Liss, Inc .