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Multi‐generational carcinogenesis from diethylstilbestrol investigated by blastocyst transfers in mice
Author(s) -
Walker Bruce E.,
Kurth Lori A.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910610218
Subject(s) - offspring , ovary , biology , diethylstilbestrol , blastocyst , andrology , pregnancy , medicine , embryo , endocrinology , estrogen , embryogenesis , genetics
Previous studies have shown that a carcinogenic effect can be transmitted from female mice exposed prenatally to diethylstil‐bestrol (DES) to their female offspring. Furthermore, male mice exposed pre‐natally to DES can transmit a carcinogenic effect to their offspring through their germ cells. To study how multi‐generational carcinogenesis is transmitted through females exposed pre‐natally to DES. the technique of blastocyst transfer was utilized. Blastocysts from strain CD‐1 mice exposed pre‐natally to vehicle were transferred to mice exposed pre‐natally to DES. Among 143 offspring from these transfers, there were 10 ovarian adenomas and 10 uterine adenocarcino‐mas. Among 92 offspring from blastocyst transfers between mice exposed pre‐natally to vehicle only, there was 1 ovarian adenoma and 1 uterine adenocarcinoma. Thus the pre‐natal exposure of the host to DES produced a maternal environment which increased the incidence of ovarian and uterine tumors. The reverse type of transfer was also performed, in which blastocysts from female mice exposed pre‐natally to DES were transferered into mice exposed to vehicle only pre‐natally. Among 99 offspring derived from DES‐exposed germ cells, 6 developed ovarian adenomas and 16 developed uterine adeno‐carcinomas. Thus DES also has a multi‐generational effect transmitted through the blastocyst, which is consistent with fetal germ cell mutation from DES. © 1995 Wiley‐Liss, Inc .