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NM23 gene expression correlates with cell growth rate and S‐phase
Author(s) -
Caligo Maria A.,
Cipollini Giovanna,
Fiore Lisa,
Calvo Simonetta,
Basolo Fulvio,
Collecchi Paola,
Ciardiello Fortunato,
Pepe Stefano,
Petrini Mario,
Bevilacqua Generoso
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600619
Subject(s) - gene expression , gene , biology , genetics
Two human NM23 genes have been identified: NM23 .HI and NM23 .H2 coding for the A and B subunit of a nucleoside diphosphate kinase (NDPK), respectively. NM23 .HI gene has been proposed as a suppressor of metastatic ability in tumor cells, NM23 .H2 is identical to the c‐myc transcription factor, PuF. The NM23 coding sequence is strongly preserved through different species. Indirect evidence of various types has been accumulated and seems to support an implication of NM23 in cell proliferation. This report shows that the NM23 gene expression is strictly related to the growth state of the cells. Two different in vitro systems (human peripheral blood lymphocytes and human breast epithelial cell line MCF‐ 10A) and one in vivo (human primary infiltrating ductal breast carcinomas) system have been investigated. The mRNA is present in PHAstimulated peripheral blood lymphocytes, whereas it is nearly undetectable in their resting counterparts. The level of the NM23 gene expression parallels the fraction of cells incorporating thymidine (S‐phase) in neoplastic mammary tissues. In synchronously cycling MCF‐10A cells NM23 .HI mRNA reaches a maximum abundance in the S‐phase and is absent or only present at very low levels during G 0 /G 1 phase, whereas NM23 .H2 is present in growth‐ arrested cells but is upregulated following serum growth stimulation.