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Expression of tissue‐type transglutaminase correlates positively with metastatic properties of human melanoma cell lines
Author(s) -
van Groningen Jan J. M.,
Klink Steve L.,
Bloemers Henri P. J.,
Swart Guido W. M.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600319
Subject(s) - tissue transglutaminase , cell culture , melanoma , northern blot , western blot , peptide , cancer research , glutamine , lysine , cell , blot , enzyme , messenger rna , rna , biology , microbiology and biotechnology , chemistry , biochemistry , amino acid , gene , genetics
In this study the relationship between tissue‐type transglutaminase (TGase2) activity and the propensity to metastasize was investigated in human melanoma cell lines with different metastatic behavior. TGase2 catalyzes an acyl‐transfer reaction between peptide‐bound glutamine residues and primary amines, including the ε‐amino group of lysine residues. Northern‐blot analysis demonstrated that TGase2 RNA‐expression (3.7 kb) was elevated in highly metastatic cell lines (MV3 and BLM) as compared to weakly metastatic ones (I F6 and 530). Immunopre‐cipitation and enzyme assays of TGase2 showed that the differential expression at the mRNA level was also reflected at the protein level. These findings reveal a positive relation between the expression of TGase2 and the metastatic properties of the human melanoma cell lines. © 1995 Wiley‐Liss, Inc.