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Human high molecular weight‐melanoma associated antigen mimicry by mouse anti‐idiotypic MAb MK2‐23. Immunohistochemical analysis of the reactivity of anti‐anti‐idiotypic MAb with surgically removed melanoma lesions
Author(s) -
Kageshita Toshiro,
Hirai Shunji,
Ono Tomomichi,
Ferrone Soldano
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600310
Subject(s) - monoclonal antibody , antigen , melanoma , molecular mimicry , immunohistochemistry , idiotype , immunotherapy , antibody , biology , microbiology and biotechnology , immune system , monoclonal , epitope , immunology , cancer research
The mouse anti‐id MAb MK2‐23 bears the internal image of the antigenic determinant defined by anti‐HMW‐MAA MAb 763.74. Active specific immunotherapy with anti‐id MAb MK2‐23 is associated with a statistically significant prolongation of survival in patients with malignant melanoma. Characterization of the immune response elicited by anti‐id MAb MK2‐23 may contribute to our understanding of the mechanisms underlying the apparent beneficial clinical effects of active specific immunotherapy with anti‐id MAb MK2‐23. Therefore, 8 HMW‐MAA binding anti‐anti‐id MAbs elicited with MAb MK2‐23 were characterized in their reactivity with a large panel of surgically removed benign and malignant tumors and of normal tissues. The 8 anti‐anti‐id MAbs displayed subtle differences in their immunoperoxidase staining of both benign and malignant lesions and of normal tissues. The diversity in the fine specificity of the 8 anti‐anti‐id MAbs is likely to reflect the few somatic mutations which occur in the amino‐acid sequence of the variable regions of their heavy and light chains in the course of the immune response to MAb MK2‐23. The reactivity patterns of the 8 anti‐anti‐id MAbs with the tissue substrates are similar, although not superimposable upon that of anti‐HMW‐MAA MAb 763.74 elicited with melanoma cells. This difference may reflect the imperfect mimicry by anti‐id MAb MK2‐23 of the antigenic determinant defined by anti‐HMW‐MAA MAb 763.74. © 1995 Wiley‐Liss, Inc.