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Loss of chromosome 3p arm differentiating tumorigenic from non‐tumorigenic cells derived from the same SV40‐transformed human mammary epithelial cells
Author(s) -
Lebeau Jérocme,
GerbaultSeureau Michèle,
Lemieux Nicole,
Apiou Francloise,
Calvo Fabien,
Berthon Philippe,
Goubin Gerard,
Dutrillaux Bernard
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600219
Subject(s) - karyotype , biology , ploidy , chromosome , cell culture , microbiology and biotechnology , cytogenetics , genetics , cancer research , gene
After immortalization of human normal mammary epithelial cells by replication‐defective SV40 genome integration, 2 cultures were developed independently. Both had the same integration site, in band 9q21, but rapidly diverged karyotypically. After a few passages, one, designated SC2T2, exhibited near‐diploid (a) and the other, designated SL2T2, near‐tetraploid (b) karyotypes. The simplest formulas were 44, X, ‐X, der(3;22) (q10;q10), der(4)t(4;9)(q34;q12), +8, +9, add(13)(pl), der(19) t(8; 19)(q21;p 13.3), add(22)(p 1) for karyotype (a) and 93, XXXX, add(1)(q12), add(11)(q13), +20 for karyotype (b). A number of alterations were further acquired with passages. Both cell cultures were tumorigenic, but their efficiency of grafting in nude mice largely differed: it was low for SL2T2 and high for SC2T2 cultures. All cultures of the xenografted tumors, obtained from either SL2T2 or SC2T2, exhibited the same clonal anomalies as those characterizing karyotype (a). It was concluded that only cells with karyotype (a) were tumorigenic, and that the difference in the tumorigenic potential of cultures SC2T2 and SL2T2 was related to their richness in cells with this karyotype. The comparison of the various karyotypes, together with data obtained in other cell types transformed by SV40, suggests that the acquisition of tumorigenicity in S2T2 mammary epithelial cells may be related to the loss of chromosome 3p arm. © 1995 Wiley‐Liss, Inc.