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Correlation between viral dna load and serum anti p19 antibody concentrationtn symptomless human T‐lymphotropic virus type‐I (HTLV‐I)‐infected individuals
Author(s) -
MorandJoubert Laurence,
Mariotti Martine,
Reed Donna,
Petit JeanClaude,
Lefrere JeanJacques
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600203
Subject(s) - antibody , titer , virology , virus , antibody titer , viral load , western blot , polymerase chain reaction , immunology , biology , dot blot , microbiology and biotechnology , viral disease , human t lymphotropic virus , dna , gene , genetics , neuroscience , myelopathy , spinal cord
Abstract In order to determine whether serum anti‐human T‐cell lymphotropic virus type I (HTLV‐I) antibody concentration is correlated with cellular viral DNA load, these 2 biological parameters were established in 22 symptomless HTLV‐I carriers. The proviral copy (PVC) number was determined through quantificative polymerase chain reaction. Specific antibody titers were determined by Western blot with the end‐point dilution method; the quantification of each antibody was performed through ScanGlot by determination of the peak height of each Western‐blot band. A positive correlation was observed between the PVC number and the titer of total antibodies. When the association between the peak height of each antibody and the PVC number was studied, a significant positive correlation was observed only with anti‐p 19. Further evaluation through follow‐up studies of symptomless HTLV‐I individuals is needed to clarify the value of anti‐HTLV‐l antibody titer as a predictor of disease progression. © 1995 Wiley‐Liss, Inc.