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nmb , a novel gene, is expressed in low‐metastatic human melanoma cell lines and xenografts
Author(s) -
Weterman Marian A. J.,
Ajubi Nasser,
van Dinter Irma M. R.,
Degen Winfried G. J.,
van Muijen Goos N. P.,
Ruiter Dirk J.,
Bloemers Henri P. J.
Publication year - 1995
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910600111
Subject(s) - complementary dna , biology , melanoma , cell culture , clone (java method) , microbiology and biotechnology , transfection , suppression subtractive hybridization , cdna library , gene , cancer research , gene expression , metastasis , cancer , genetics
From a subtractive cDNA library, we isolated several cDNA clones which showed differential expression between highly and lowly metastatic human melanoma cell lines. One clone, designated nmb , showed preferential expression in the low‐meta‐static cell lines and was chosen for further characterization. Sequence analysis revealed that this clone represents a novel gene, encoding a putative transmembrane glycoprotein which showed the highest homology to the precursor of pMEL17, a melanocyte‐specific protein. nmb RNA expression was absent in most tumor‐cell lines tested and not restricted to the melanocytic lineage. Transfection of a partial nmb cDNA into a highly metastatic melanoma cell line (BLM) resulted, in 2 of 3 transfectants, in slower subcutaneous tumor growth and, in 1 of 3 transfectants, in reduction of the potential for spontaneous metastasis in nude mice. © 1995 Wiley‐Liss, Inc.