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Chimeric transcripts with an open reading frame are generated as a result of translocation to the Pvt‐1 region in mouse B‐cell tumors
Author(s) -
Huppi Konrad,
Siwarski David
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590623
Subject(s) - biology , open reading frame , chromosomal translocation , microbiology and biotechnology , breakpoint , locus (genetics) , genetics , rna splicing , allelic exclusion , rna , gene , peptide sequence , t cell , t cell receptor , immune system
Some mouse plasmacytomas exhibit a t(6;15) chromosomal translocation in which the breakpoint resides within the Pvt‐1 locus located 260 kilobases (kb) downstream of c‐ myc on mouse chromosome 15. In this report, we show that the Pvt‐1 locus does not exhibit allelic exclusion in that Pvt‐1 transcripts continue to be expressed from the non‐translocated allele in t(6;15) plasmacytomas. From the translocated allele, we find chimeric transcripts containing a short 57‐bp segment of Pvt‐1 (termed Pvt‐1a ) spliced directly to the immunoglobulin constant region sequence ( lg‐Ck ). These short transcripts have replaced a Jk segment with a trytophan residue via RNA splicing and contain a continuous open reading frame (ORF) from Pvt‐1a through Ck . Since this Pvt‐1a/Ck transcript is found in all 3 t(6;15) plasmacytomas examined, regardless of the location of the chromosomal breakpoint, we suggest that the Pvt‐1a/Ck chimera may have a functional role in the development of mouse plasmacytomas.