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Expression of the FGFR1 gene in human breast‐carcinoma cells
Author(s) -
Jacquemier Jocelyne,
Adelaide José,
Parc Patricia,
PenaultLlorca Frédérique,
Planche Jacqueline,
Delapeyriere Odile,
Birnbaum Daniel
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590314
Subject(s) - fibroblast growth factor receptor 1 , biology , northern blot , cancer research , gene , gene expression , fibroblast growth factor , receptor tyrosine kinase , breast carcinoma , karyotype , in situ hybridization , tyrosine kinase , messenger rna , chromosome , microbiology and biotechnology , receptor , breast cancer , cancer , genetics
Fibroblast growth factors (FGF) constitute a family of at least 9 members which act through high‐affinity tyrosine‐kinase receptors encoded by 4 distinct genes. In humans, the FGFRI gene is located in chromosomal region 8pl2. Its amplification and expression were examined in a panel of 110 breast carcinoma samples by Southern‐ and Northern‐blot analyses. FGFRI was amplified in 9% and overexpressed in about 15% of the tumors studied. In situ hybridization experiments were performed on tissue sections of normal breast and tumors with a high level of FGFRI expression. In both normal and tumoral tissues, FGFRI RNA was detected in the epithelial cells. Overexpression of FGFRI seems to be associated with small, well‐differentiated diploid tumors. © 1994 Wiley‐Liss, Inc.