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Expression of MUC2‐mucin in colorectal adenomas and carcinomas of different histological types
Author(s) -
Blank Monika,
Klussmann Enno,
KrügerKrasagakes Sabine,
SchmittGräff Anette,
Stolte Manfred,
Bornhoeft Gudrun,
Stein Harald,
Xing PeiXiang,
McKenzie Ian F. C.,
Verstijnen Cees P. H. J.,
Riecken Ernst Otto,
Hanski Christoph
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590302
Subject(s) - mucin 2 , mucin , immunohistochemistry , pathology , adenocarcinoma , carcinoma , adenoma , mucinous carcinoma , biology , muc1 , cancer , medicine , gene expression , gene , biochemistry
The expression of mucin MUC2 was investigated in normal colonic tissue, in colonic adenomas and in carcinomas of the mucinous and non‐mucinous type. The latter were subdivided into carcinomas originating from the adenoma‐carcinoma sequence (ACS) and de novo (DN) carcinomas. The expression was assayed by immunohistochemistry with the monoclonal anti‐MUC2 antibody CCP58 and by mRNA semiquantitation. MUC2 protein epitope CCP58 was strongly expressed in 21 % of normal colonic tissues, in 40% of villous and in 48% of tubular adenomas. Mucinous carcinomas exhibited strong expression in 72%, ACS carcinomas in 21 % and DN adenocarcinomas in none of the tumors investigated. Compared with the adjacent non malignant tissue (transitional mucosa), CCP58 epitope expression in the tumor was higher in 74% of mucinous carcinomas, but equal or lower in 69% of ACS carcinomas and in 100% of de novo carcinomas. The alterations of MUC2 expression detected by immunohistochemistry in adenocarcinomas were confirmed on mRNA level. These data indicate that the MUC2 expression pattern is different in the 3 carcinoma types investigated. MUC2 over‐expression occurs in the adenomatous tissue. It is always maintained in mucinous carcinomas, but frequently decreased in non‐mucinous ACS carcinomas. DN carcinomas are most frequently associated with decreased expression of MUC2. © 1994 Wiley‐Liss, Inc.