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Induction of multidrug resistance (MDR) by transfection of MCF‐10A cell line with c ‐Ha‐ ras and c‐erbB‐2 oncogenes
Author(s) -
Sabbatini Antonietta R. M.,
Basolo Fulvio,
Valentini Paola,
Mattii Letizia,
Calvo Simonetta,
Fiore Lisa,
Ciardiello Fortunato,
Petrini Mario
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590212
Subject(s) - multiple drug resistance , transfection , mcf 7 , cell culture , p glycoprotein , cancer research , oncogene , biology , microbiology and biotechnology , verapamil , phenotype , cell , drug resistance , medicine , gene , cell cycle , cancer , cancer cell , human breast , biochemistry , genetics , calcium
To investigate the relationship between oncogene activation and appearance of multidrug resistance (MDR) we transfected the human breast epithelial cell line MCF‐10A, negative for the expression of the P‐glycoprotein, with c ‐Ha‐ ras and/or c‐erbB‐2 oncogenes. The appearance of the MDR phenotype was then studied by evaluating mdr‐1 mRNA expression, the presence of P‐glycoprotein on the cell membrane and the onset of doxorubicin resistance, together with the effect of the reversing agent verapamil. We found that only MCF‐10A transfected with both c ‐Ha‐ ras and c‐erbB‐2 oncogenes acquired the MDR phenotype.