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Mutations of the adenomatous polyposis coli gene in the mutation cluster region: Comparison of human pancreatic and colorectal cancers
Author(s) -
Yashima Kazuo,
Nakamori Shouji,
Murakami Yoshinori,
Yamaguchi Akio,
Hayashi Kenshi,
Ishikawa Osamu,
Konishi Youichi,
Sekiya Takao
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590110
Subject(s) - adenomatous polyposis coli , gene , mutation , gene mutation , colorectal cancer , familial adenomatous polyposis , cancer research , biology , polymerase chain reaction , mutation frequency , genetics , cancer
We analyzed the adenomatous polyposis coli ( APC ) tumorsuppressor gene as one of the possible genes mutated in human pancreatic carcinomas. DNAs from 39 surgical specimens were subjected to single‐strand conformation polymorphism analysis of polymerase chain reaction products and the mutation cluster region (MCR) of the gene was examined. We also examined the same region of DNAs from 27 surgical specimens of sporadic colon carcinomas and detected mutations in 11 carcinomas (41%). This mutation frequency in colon carcinomas was similar to those reported previously. Using this system, we detected a mutated APC gene in one of 39 pancreatic carcinomas. The results indicated that mutation of the MCR in the APC gene is involved in genesis of some of human pancreatic carcinomas, but its frequency is much lower than in colorectal carcinomas.