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ras ‐family gene mutations in neoplasia of the ampulla of vater
Author(s) -
Scarpa Aldo,
Zamboni Giuseppe,
Achille Alberto,
Capelli Paola,
Bogina Giuseppe,
Iacono Calogero,
Serio Giovanni,
Accolla Roberto S.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910590109
Subject(s) - ampulla of vater , ampulla , biology , adenoma , exon , carcinoma , gene , bile duct , adenocarcinoma , cancer research , pathology , gastroenterology , medicine , cancer , genetics , anatomy
Mutations in the first and second exons of Ha‐, Ki‐ and N‐ ras oncogenes were investigated in 17 epithelial tumors of the ampulla of Vater by single‐strand conformation polymorphism analysis and direct sequencing of DNA fragments amplified by polymerase chain reaction. The panel included 12 intestinaltype adenocarcinomas, 3 villous adenomas, I papillary carcinoma and I neuroendocrine carcinoma. Six cases (35%) contained ras mutations, affecting codon 12 of Ki‐ ras in 2 adenomas and 3 carcinomas, and of N‐ ras in I adenoma. All mutations were found in adenomas and among cancers with adenomatous areas, whereas none of the cases lacking adenomatous areas contained mutations. This suggested that ampullary cancers represent heterogeneous diseases with respect to the presence or absence of adenomatous areas and, among those with adenomatous areas, with respect to the presence of activated ras genes. Ki‐ ras mutated cases included 3 of 4 tumors which mainly involved the intraduodenal bile duct, thus suggesting that a proportion of Ki‐ ras ‐mutated ampullary cancers might correspond to those originating from the epithelium of the bile duct component of the ampulla.