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Sulfonated aluminium phthalocyanines as sensitizers for photochemotherapy. Effects of small light doses on localization, dye fluorescence and photosensitivity in V79 cells
Author(s) -
Moan Johan,
Berg Kristian,
Anholt Helle,
Madslien Kari
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580620
Subject(s) - photosensitivity , fluorescence , acridine orange , photochemistry , chemistry , biophysics , phthalocyanine , staining , phototoxicity , biochemistry , materials science , biology , in vitro , pathology , medicine , optics , organic chemistry , optoelectronics , apoptosis , physics
Abstract V79 cells incubated with di‐ or tetrasulfonated aluminium phthalocyanines (AIPcS 2 or AIPcS 4 ) showed a granular fluorescence pattern. Co‐staining with the lysosomotropic dye acridine orange (AO) indicated that the granules that were stained by these photoactive phthalocyanines were identical to lysosomes. Small light exposures made the lysosomes permeable to the dyes without inactivating the cells. Also, the lysosomal enzymes β‐AGA and cathepsin (L + B) were inactivated by small light exposures when AIPcS 4 was present. Such small and almost nontoxic light exposures caused a redistribution of the dyes in the cells that was accompanied by a more than 10‐fold increase in the fluorescence quantum yields of the dyes. Surprisingly, this redistribution and increase in fluorescence did not result in any significant increase in the photosensitivity of the cells.