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The chondroitin sulfate proteoglycan NG2 is a tumour‐specific antigen on the chemically induced rat chondrosarcoma HSN
Author(s) -
Léger Olivier,
JohnsonLéger Caroline,
Jackson Elizabeth,
Coles Brian,
Dean Christopher
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580514
Subject(s) - chondroitin sulfate proteoglycan , complementary dna , amino acid , microbiology and biotechnology , biology , chondroitin sulfate , biochemistry , proteoglycan , oligonucleotide , peptide sequence , signal peptide , glycoprotein , antigen , transmembrane protein , transmembrane domain , extracellular matrix , dna , gene , glycosaminoglycan , immunology , receptor
N‐terminal sequences of 19 and 11 amino acids obtained from 2 different Cryptic fragments of the tumour‐specific antigen on the chemically induced rat chondrosarcoma HSN show a 100% homology with the rat chondroitin sulfate proteoglycan NG2. Using a scheme of overlapping oligonucleotide primers we have cloned by PCR amplification the cDNA for the specific antigen of the HSN tumour that is immunogenic in immunocompetent CBH/Cbi rats and now report that its cDNA sequence is identical to that of NG2. The cDNA codes for a transmembrane protein of 2,325 amino acids with a large extracellular domain of 2,224 amino acids containing 2 cysteine‐rich regions, a trans‐membrane domain (25 amino acids) and a short cytoplasmic tail (76 amino acids). The tumour‐specific determinant was found to lie between amino acid residues 556 and 992 on the core glycoprotein.