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Molecular analysis of the retinoblastoma gene in primary ovarian cancer cells
Author(s) -
Liu Yie,
Hbyman Mats,
Wang Yisong,
Falkmer Ursula,
Hising Christina,
Székely László,
Einhorn Stefan
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580508
Subject(s) - ovarian cancer , loss of heterozygosity , retinoblastoma , locus (genetics) , allele , exon , gene , cancer research , biology , malignant transformation , tumor suppressor gene , pathology , cancer , genetics , medicine , carcinogenesis
Loss of heterozygosity (LOH) at the site of the retinoblastoma (RBI) gene, at 13ql4, has been shown to occur in a high proportion of ovarian cancer patients. Based on this, RBI gene mactivation was studied in primary tumor cells from 15 patients with ovarian cancer. Structural changes as well as expression of the RBI gene were investigated. One patient had a noncisiunctional deletion at the RBI locus, and the duplicated smaining allele carried a deletion of exon 21. In another patient, without detectable structural changes of the RBI gene. no RB protein was detected. Allelic losses at the RBI locus were observed in 8/13 informative cases (61%). Our results indicate that inactivation of the RBI gene plays a role in tumor development in a minority of ovarian cancer patients. Another gene(s) on 13q also seem(s) to influence malignant transformation in patients with ovarian cancer.