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Cloning of feline p53 tumor‐suppressor gene and its aberration in hematopoietic tumors
Author(s) -
Okuda Masaru,
Umeda Akiko,
Sakai Tadashi,
Ohashi Takashi,
Momoi Yasuyuki,
Youn HwaYoung,
Watari Toshihiro,
Goitsuka Ryo,
Tsujtmoto Hajimc,
Hasegawa Atsuhiko
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580425
Subject(s) - cloning (programming) , tumor suppressor gene , cancer research , haematopoiesis , gene , biology , suppressor , pathology , medicine , carcinogenesis , stem cell , genetics , computer science , programming language
Alterations of the p53 tumor‐suppressor gene have been observed in a variety of human and mouse tumors. For investigation of the role of this gene in tumors of cats, feline p53 cDNA was molecularly cloned from a feline lymph‐node cDNA library. The cloned cDNA (FF53) contained the whole open reading frame of p53 gene encoding 386 amino acids. The amino‐acid sequence of the feline p53 gene showed 82.1% and 74.9% similarities with those of the human and mouse counterparts, respectively, and had structural characteristics in common with the p53 genes of several other species. Aberrations of the p53 gene were investigated by RT‐PCR and single‐strand conformation polymorphism analyses. Of 10 primary hematopoietic tumors and 3 lymphoma cell lines examined, one lymphoma and one lymphoma cell line had a point mutation of the p53 gene, resulting in single amino‐acid substitutions.