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A K‐ ras 13GLY → ASP mutation is recognized by HLA‐DQ7 restricted T cells in a patient with colorectal cancer. Modifying effect of DQ7 on established cancers harbouring this mutation?
Author(s) -
Fossum Beate,
Breivik Jarle,
Meling Gunn Iren,
Iii Tobias GeddeDahl,
Hansen Torbjørn,
Knutsen Ingebjørg,
Rognum Torleiv O.,
Thorsby Erik,
Gaudernack Gustav
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580409
Subject(s) - mutation , colorectal cancer , cancer , cancer research , medicine , epitope , human leukocyte antigen , immunology , microbiology and biotechnology , biology , antigen , genetics , gene
We have characterized and described in detail 2 CD4 + T‐lymphocyte clones (TLC) from a colonic cancer patient. These TLC specifically recognize a K‐ ras ‐derived peptide carrying the 13Asp mutation commonly found in adenocarcinomas of the colon. The TLC were independently derived, as they carried 2 different T‐cell receptors. The TLC recognized partly overlapping epitopes within the 13Asp peptide, presented by HLA‐DQ7 molecules, suggesting that this molecule might confer some protective immunity against the mutation. On the basis of analysis of 251 colonic carcinomas, the presence of HLA‐DQ7 did not seem to protect against the establishment of carcinomas carrying the 13Asp mutation, since the frequency of the DQ7 haplotype was not decreased among patients having this mutation. A modifying effect of DQ7 on the development of carcinomas with a 13Asp mutation was, however, observed, resulting in fewer tumours reaching advanced Dukes stages when DQ7 was present.