Premium
Expression of transforming growth factor‐beta (TGF‐β) receptors, TGF‐β1 and TGF‐β2 production and autocrine growth control in osteosarcoma cells
Author(s) -
Kloen Peter,
Jennings Candace L.,
Gebhardt Mark C.,
Springfield Dempsey S.,
Mankin Henry J.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910580323
Subject(s) - autocrine signalling , transforming growth factor , osteosarcoma , cell growth , r smad , cell culture , transforming growth factor beta , biology , cancer research , growth factor , receptor , cell , cell cycle , medicine , microbiology and biotechnology , endocrinology , tgf alpha , biochemistry , genetics
Transforming growth factor‐beta (TGF‐β) is a polypeptide with multiple physiological functions. Isoforms of this growth factor have important roles in control of the cell cycle, in regulation of cell‐cell interactions and in growth and development. Malignant transformation has been shown to be associated with increased expression of TGF‐β. Since bone is the largest storage site and producer of TGF‐β, we speculated on the existence of an autocrine mechanism in osteosarcoma, a malignant bone tumor. Expression of TGF‐β cell surface receptors, effects on growth of TGF‐p and TGF‐β antibodies and production of 2 TGF‐β isoforms were studied in a panel of 7 osteosarcoma cell lines. In contrast to most previous reports on the effects of TGF‐β on osteosarcoma cell growth, we found a mitogenic effect of TGF‐β 1 in 4 of 7 osteosarcoma cell lines. Receptor profiles for TGF‐β were aberrant in 5 of the 7 cell lines tested, and production of TGF‐β1 and TGF‐β2 varied among cell lines. Addition of anti‐TGF‐β antagonized the effects of endogenous TGF‐β. Our results suggest a potential role of TGF‐β in autocrine growth control of osteosarcoma cells.