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Chromosome aberrations in adenomas of the colon. Proof of trisomy 7 in tumor cells by combined interphase cytogenetics and immunocytochemistry
Author(s) -
Herbergs J.,
de Bruïne A. P.,
Marx P. T. J.,
Vallinga M. I. J.,
Stockbrügger R. W.,
Ramaekers F. C. S.,
Arends J. W.,
Hopman A. H. N.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570604
Subject(s) - cytogenetics , trisomy , immunocytochemistry , pathology , biology , chromosome , medicine , microbiology and biotechnology , genetics , gene
Thirty‐five colon adenomas from 26 patients were analyzed with centromeric probes for chromosomes 1,7, 17, X and Y in order to study numerical aberrations, chromosome imbalances, aneuploidy and tetraploidization. The fluorescent in situ hybridization (FISH) technique was applied to single‐cell suspensions and a combination of FISH and immunocytochemistry (ICC) was employed to identify the cell type under study. Trisomy of chromosome 7 was detected in 37% of the cases. In 7 out of 13 cases this aberration was combined with abnormalities of one or 2 of the other investigated chromosomes. No correlation could be demonstrated between any of the detected chromosomal aberrations and size, localization or degree of epithelial dysplasia. With the combined FISH/ICC procedure, the abnormal cells were shown to be of epithelial rather than of stromal origin. Our data indicate that trisomy 7 is a common chromosome aberration in the epithelial component of colon adenomas. © 1994 Wiley‐Liss, Inc.