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Study of the resistance of tumor‐cell spheroids to penetration and lysis by activated effector cells
Author(s) -
Ochalek Tomasz,
von Kleist Sabine
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570318
Subject(s) - effector , cytotoxic t cell , lymphokine activated killer cell , lymphocyte , lysis , in vitro , spheroid , biology , t lymphocyte , cell , immunology , cytotoxicity , cancer research , chemistry , microbiology and biotechnology , interleukin 12 , biochemistry
Tumor spheroids and growing cell monolayers were used as 2‐ and 3‐dimensional targets in the in vitro study of the interactions of colorectal tumor cells with various in vitro II‐2‐activated lymphocyte effectors. The study examined tumor‐cell susceptibility to the cytotoxic activity of effector cells in correlation with the ability of the effectors to infiltrate spheroids. No restriction of lymphocyte activity was found when the susceptibility of tumor cells to the panel of autologous and allogeneic II‐2‐stimulated PBL (LAK cells) and TIL was compared. Their activity against a given tumor specimen was similar, but differed against the various other tumor specimens. Thus, the tumor susceptibility and not the cytotoxic potential of the lymphocytes determined the interaction result. An inhibition of lymphocyte penetration was observed in some tumor spheroids, which considerably influenced the resistance to lymphocyte lysis. Apparently it is this inhibition of the migratory capacity of the effector cells by tumor cells that is responsible for tumor resistance to lymphocyte attack. © 1994 Wiley‐Liss, Inc.