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Partial characterization of a cDNA for human stearoyl‐CoA desaturase and changes in its mRNA expression in some normal and malignant tissues
Author(s) -
Li Juan,
Ding ShiFa,
Habib Nagy A.,
Fermor Beverley F.,
Wood Chris B.,
Gilmour R. Stewart
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570310
Subject(s) - complementary dna , messenger rna , biology , microbiology and biotechnology , gene , hepatocellular carcinoma , gene expression , polymerase chain reaction , reverse transcriptase , rna , coding region , biochemistry , genetics
A segment of 712 bases coding for part of the human stearoyl‐CoA desaturase gene was made by polymerase chain reaction (PCR) using primers based on published rat cDNA sequences. The human PCR product was confirmed by DNA sequencing. It was next cloned into a vector from which anti‐sense, highly radioactive RNA transcripts were made in vitro using T 7 polymerase. The transcripts were used to probe desaturase mRNA in a number of human tumour and control tissues, using a very sensitive solution hybridization/RNase protection assay. Increased desaturase mRNA levels were found in colonic and oesophageal carcinomas and in hepatocellular adenoma; however, no consistent trend was seen in hepatocellular carcinoma. It is suggested that certain classes of tumour may exhibit increased levels of desaturase mRNA. © 1994 Wiley‐Liss, Inc.