Premium
Suppressive effect of recombinant human Cu, Zn‐superoxide dismutase on lung metastasis of murtne tumor cells
Author(s) -
Yoshizaki Naohito,
Mogi Yoshihiro,
Muramatsu Hirohito,
Koike Kazuhiko,
Kogawa Katsuhisa,
Niitsu Yoshiro
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570226
Subject(s) - superoxide dismutase , metastasis , recombinant dna , lung , human lung , cancer research , chemistry , medicine , biochemistry , cancer , enzyme , gene
The inhibitory effect of recombinant human Cu ++ Zn ++ superoxide dismutase (rhSOD) on metastasis of tumor cells in the mouse was investigated. In an experimental pulmonary metastasis model employing Moth A cells as inoculum, significant inhibition of metastasis was obtained by intravenous pre‐ and post‐administration of rhSOD. An inhibitory effect of rhSOD was also observed in a spontaneous pulmonary metastasis model with ILL cells as the inoculum. rhSOD was not observed to have any significant effects on the platelet‐aggregating activity of tumor cells, the adhesiveness of tumor cells to vascular components (endothelial cells, laminin and type‐IV collagen), or the growth of tumor cells either in vitro or in vivo. However, rhSOD suppressed invasion of Meth A and 3LL cells into Matrigel (an artificially reconstituted basement membrane of collagen, laminin and heparan sulfate) in the presence of hypoxanthine and xanthine oxidase, in vitro producers of superoxide. Thus, the present study shows that rhSOD is able to inhibit both experimental and spontaneous pulmonary metastasis, possibly through the suppression of tumor cell invasion into the extracellular matrix. © 1994 Wiley‐Liss, Inc.