Quercetin enhances transforming growth factor β 1 , secretion by human ovarian cancer cells | Zendy

Scambia Giovanni | Zendy; Panici Pierluigi Benedetti | Zendy; Ranelletti F. O. | Zendy; Ferrandina G. | Zendy; de Vincenzo R. | Zendy; Piantelli M. | Zendy; Masciullo V. | Zendy; Bonanno G. | Zendy; Isola G. | Zendy; Mancuso S. | Zendy

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Quercetin enhances transforming growth factor β 1 , secretion by human ovarian cancer cells

Author(s) -

Scambia Giovanni,

Panici Pierluigi Benedetti,

Ranelletti F. O.,

Ferrandina G.,

de Vincenzo R.,

Piantelli M.,

Masciullo V.,

Bonanno G.,

Isola G.,

Mancuso S.

Publication year - 1994

Publication title -

international journal of cancer

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.475

H-Index - 234

eISSN - 1097-0215

pISSN - 0020-7136

DOI - 10.1002/ijc.2910570214

Subject(s) - transforming growth factor beta , endocrinology , autocrine signalling , transforming growth factor , clonogenic assay , secretion , medicine , tgf beta signaling pathway , cell culture , beta (programming language) , biology , cell growth , receptor , growth factor , northern blot , cancer research , gene expression , biochemistry , gene , computer science , genetics , programming language

Our study demonstrates that quercetin (Q)‐induced growth‐inhibitory activity in ovarian cancer cells may be mediated by modulation of transforming growth factor β 1 (TGFβ 1 ) production. We used the OVCA 433 cell line which is very sensitive to the anti‐proliferative effect of Q and expresses high‐affinity, low‐capacity TGFβ 1 receptors. Conditioned medium (CM) from Q‐treated cells is able to displace 125I ‐TGFβ 1 from binding to its receptor; moreover Q (10 μM) increases TGFβ 1 activity in CM in a time‐dependent fashion starting after 4 hr and reaching a maximum by 24 hr of Q treatment. Q‐induced growth inhibition is reversed by a neutralizing anti‐TGFβ 1 MAb both in OVCA 433 and in a clonogenic assay of cells from a primary ovarian tumor. Q‐induced increase of TGFβ 1 activity in CM is specific since other anti‐proliferative compounds, such as Dexamethasone, which is as active on the cell cycle as Q, had no effect on TGFβ 1 secretion. Northorn‐blot analysis of TGFβ 1 mRNA levels at various times of Q (10 μM) exposure revealed that there was no increase, suggesting that regulation of TGFβ 1 occurs at post‐transcription at levels. © 1994 Wiley‐Liss, Inc.

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