z-logo
Premium
Multiple loci on human chromosome 11 control tumorigenicity of BK virus transformed cells
Author(s) -
Sabbioni Silvia,
Negrini Massimo,
Possati Laura,
Bonfatti Alessandra,
Corallini Alfredo,
Sensi Alberto,
Stanbridge Eric J.,
BarbantiBrodano Giuseppe
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570210
Subject(s) - biology , chromosome , carcinogenesis , phenotype , papovavirus , gene , tumor suppressor gene , positional cloning , genetics , suppressor , virus , neoplastic transformation , microbiology and biotechnology , cancer research
BK virus (BKV) is a human papovavirus that readily transforms rodent cells, but not human cells, to a neoplastic phenotype, suggesting that tumor‐suppressor functions expressed in human cells control BKV oncogenicity. Transfer of a normal human chromosome 11 to BKV‐transformed mouse cells suppresses the malignant phenotype. In this report we map the regions of chromosome involved in tumor suppression. Transfer of chromosome 11 to the BKV‐transformed hamster cell line HKBK produces monochromosomic hybrids retaining only portions of the transferred human chromosome. We have compared the tumorigenicity of the hybrids with the molecular mapping of chromosome 11 retained regions. This analysis indicated that 3 regions of human chromosome 11, 11p15.5, 11p13 and 11q13, cooperate in tumor suppression. However, 11q13 seems the most important, since all the HKBK/H11‐induced tumors analysed had lost this region, whereas 11p15.5 and 11p13 were sometimes retained. The chromosomal regions identified in this study are deleted in several types of human tumors, suggesting that the BKV transformation system specifically detects tumor‐suppressor genes on chromosome 11 that are involved in human oncogenesis. This model may be of use in isolating and cloning such genes. The results of this report raise the possibility that BKV may have a synergistic tumorigenic effect in human cells where tumor‐suppressor genes controlling its oncogenk potential are inactivated. © 1994 Wiley‐Liss, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here