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Arachidonic acid metabolism in benign and malignant prostatic tissue in vitro : Effects of fatty acids and cyclooxygenase inhibitors
Author(s) -
Chaudry Aziz A.,
Wahle K. W. J.,
McClinton S.,
Moffat L. E. F.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910570208
Subject(s) - arachidonic acid , in vitro , cyclooxygenase , metabolism , cyp2c8 , arachidonic acid metabolism , biochemistry , chemistry , fatty acid , endocrinology , medicine , enzyme , cytochrome p450 , cyp2c9
Concentrations of fatty acids (FA) in prostatic tissue of patients with either benign or malignant prostatic disease have previously been shown to be significantly different. In particular, there was a significant reduction in arachidonic acid (AA, C20:4n‐6) and docosapentaenoic acid (DPA, C22:5n‐6) concentrations in malignant prostatic tissue (PCa) phospholipids (PL). It was suggested that the decreased AA concentration in PCa may be due to its increased metabolism via the cyclooxygenase (CO) and/or lipoxygenase (LO) pathways to produce eicosanoids such as prostaglandins (PGs) and/or leucotrienes (LTs) rather than an impairment in desaturase activity in situ. The eicosanoid production in benign prostatic tissue (BPH) and PCa was determined using [ 3 H]AA. The only eicosanoid produced in significant amounts by either tissue was PGE 2 and PCa converted radiolabelled AA to PGE 2 at an almost 10‐fold higher rate than BPH. PGE 2 production from [ 3 H]AA by PCa was investigated in the presence of oleic acid (OA, C18:In‐9), eicosapentaenoic acid (EPA, C20:5n‐3), docosahexaenoic acid (DHA, C22:6n‐3), dihomo‐gamma‐linolenic acid (DGLA, C20:3n‐6), eicosatetraynoic acid (FTYA) and ketoprofen (KPN) respectively. OA was found to be the most effective inhibitor of PGE 2 production by PCa compared with DHA. EPA, ETYA and KPN, while DGLA was the least effective. Diacyiglycerol (DAG) formation from labelled AA by PCa was about 4‐fold greater than in BPH. Such high levels of DAG may be a means of promoting tumorigenesis through activation of protein kinase C as found with phorbol esters which can be regarded as DAG analogues. © 1994 Wiley‐Liss, Inc.