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Polymorphism of T‐cell receptor genes in nasopharyngeal carcinoma
Author(s) -
Chen Yan,
Chan Soh Ha
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910560613
Subject(s) - bamhi , southern blot , nasopharyngeal carcinoma , ecori , restriction fragment length polymorphism , hindiii , biology , microbiology and biotechnology , restriction fragment , genetics , complementary dna , genomic dna , restriction enzyme , allele , gene , genotype , medicine , radiation therapy
Germ‐line TCR genes from 41 Singaporean Chinese patients with nasopharyngeal carcinoma and 54 Singapore Chinese normal subjects were investigated by restriction fragment length polymorphism (RFLP) analysis. Genomic DNA from BLCLs of patients and normal subjects was digested with the restriction end nucleases BamHI, EcoRI, HindIII, Taql, transferred to nylon membrane by the Southern‐blot technique and hybridized with radioactively labelled TCR cDNA probes. When the combination of BamHI/VβII was used, NPC patients had a lower frequency of the 25/25‐kb allelic pattern and a higher frequency of the 20‐kb fragment compared to controls. With the combination of Taql/Vβ8, the 4.3/3.2‐kb fragment allelic frequency was lower in total NPC but particularly in patients with HLA B46. The result suggested that TCR restriction may be important in the pathogenesis of NPC.