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Frequency and characterization of p53 mutations in primary and metastatic human prostate cancer
Author(s) -
Dinjens Winand N. M.,
Van Der Weiden Marcel M.,
Schroeder Fritz H.,
Bosman Fred T.,
Trapman Jan
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910560504
Subject(s) - single strand conformation polymorphism , prostate cancer , exon , lymph node , pathology , metastasis , primary tumor , immunohistochemistry , prostate , biology , mutation , prostatectomy , cancer , cancer research , gene , microbiology and biotechnology , medicine , genetics
We have studied the frequency of mutations in the p53 gene in human prostate cancer. The investigated material consisted of 20 primary‐tumor tissue specimens, obtained by transurethral resection and tissue specimens of 15 lymph‐node metastases, obtained at total prostatectomy. The applied methods encompassed immunohistochemistry on frozen sections, using the monoclonal antibody PAb 1801, and single‐strand conformation polymorphism (SSCP) analysis, after amplification of single exon sequences by PCR, on exons 5 to 8 of the p53 gene. The mutations, leading to aberrantly migrating bands in the PCRSSCP analysis, wereidentified by direct sequencing of the PCR product. Immunohistochemical and PCR‐SSCP analysis were completely confirmative. In the primary tumors, mutations were found in 10% of the specimens (codons 232 and 273), and in lymph‐node metastases in 15% of the specimens (codons 248 and 273). In one case (codon 273), the same mutation was found both in the primary tumor and in the lymph‐node metastasis. Our results show that p53 mutations are infrequent in both primary and metastatic prostate tumors. In addition, they indicate that there is no strict correlation between p53 mutation and tumor metastasis.