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Karyotypic characterization of human T‐cell lines immortalized by Herpesvirus saimiri
Author(s) -
Troidl B.,
Simmer B.,
Fickenscher H.,
MüjllerFleckenstein I.,
Emmrich F.,
Fleckenstein B.,
Gebhart E.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910560325
Subject(s) - virology , cell culture , biology , human herpesvirus , herpesviridae , human cell , virus , microbiology and biotechnology , genetics , viral disease
Merpesvirus saimiri subgroup‐C strains transform non‐neoplastic human T lymphocytes in vrtro to antigen‐independent continuous growth. The karyotypes of 8 of these T‐cell lines, derived from peripheral blood or bone marrow, are analyzed here, In general, these lines showed a normal diploid karyotype, although one of them had acquired a clonal chromosomal rearrangement after 21 months in culture. Chromosomally aberrant cell clones were found more frequently in cell lines derived from blood lymphocytes of leukemia patients than in cultures from healthy donors: However, the observed aberrations, some of which were defined in detail using chromosome in situ suppression hybridization (CISS) with chromosome‐specific recombinant ONA libraries, did not show any relation between the various cell lines nor to the respective types of leukemia.