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Cyclic AMP increases the release of parathyroid hormone‐related protein from a lung‐cancer cell line
Author(s) -
Rizzoli René,
Aubert Michel Lucien,
Sappino André Pascal,
Bonjour JeanPhilippe
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910560323
Subject(s) - calcitonin , medicine , forskolin , endocrinology , parathyroid hormone related protein , cycloheximide , trypan blue , parathyroid hormone , resorption , calcium , chemistry , reabsorption , cell culture , stimulation , biology , protein biosynthesis , cell , kidney , biochemistry , genetics
Parathyroid hormone‐related protein (PTHrP) plays an important role in the pathogenesis of malignant hypercalcemia by stimulating bone resorption and/or renal tubular reabsorption of calcium. In cultured cancer cells, its production can be influenced by various factors or ions, but the regulation of its production is still poorly understood. We investigated the effects of stimulators of cAMP synthesis on PTHrP release by a human lung squamous‐carcinoma cell line (BEN). In supervised cells grown on microcarrier beads, PTHrP production was significantly increased after incubation with calcitonin for only 20 min. The release of immunoreactive and bioactive PTHrP was increased by incubating the cells with forskolin, 3‐isobutyl‐I‐methylxanthine or dibutyryl cAMP even in the presence of the protein‐synthesis inhibitor cycloheximide for 6 hr. The calcitonin‐mediated stimulation was not accompanied by. concomitant changes in PTHrP mRNA. The microfilament‐disrupter cytocha‐lasin D was shown to enhance the basal and calcitonin‐induced production of PTHrP. These results indicate that stimulators of cAMP synthesis enhanced PTHrP release by BEN cells.