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Identification of integrated epstein‐barr virus in nasopharyngeal carcinoma using pulse field gel electrophoresis
Author(s) -
KripalaniJoshi S.,
Law H. Y.
Publication year - 1994
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910560207
Subject(s) - nasopharyngeal carcinoma , raji cell , epstein–barr virus , biology , virus , gel electrophoresis , microbiology and biotechnology , dna , virology , carcinoma , medicine , genetics , radiation therapy
Integrated Epstein‐Barr virus (EBV) was found in 4 of 17 pathologically diagnosed, EBV‐positive nasopharyngeal carcinoma (NPC) biopsy samples using pulse field gel electrophoresis (PFGE). The analyses were carried out after digestion of biopsy DNA with rare restriction enzymes such as Pacl, which does not cleave EBV due to the absence of the recognition sequence, and Clal. Employing this technique, EBV integration in IB4, through Ecol, and Namalwa through the terminal repeats, was confirmed. We also established that integration of EBV in AW Ramos was through the terminal repeats. The Raji cell line was also found to harbour integrated virus in addition to episomal DNA. However, the site of integration could not be confirmed, since as the Raji DNA appeared to be heavily methylated and could not be cleaved with C P G rare cutters. As in the Raji cell line, the DNA of EBV in nasopharyngeal‐carcinoma (NPC) biopsies also appeared to be methylated.