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Effect of serum and cell density on transmembrane distribution of cAMP and cGMP in transformed (C4‐i) and non‐transformed (WI‐38) human cells
Author(s) -
Ørbo A.,
Jaeger R.,
Sager G.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910550614
Subject(s) - intracellular , endocrinology , extracellular , medicine , cell , in vitro , cell culture , cell growth , biology , transmembrane protein , distribution (mathematics) , cell type , microbiology and biotechnology , chemistry , biochemistry , receptor , genetics , mathematical analysis , mathematics
The ratio between cGMP and cAMP in plasma/urine is elevated in several types of malignancies. The present in vitro study showed that the ratio between extracellular cGMP and cAMP increased during the proliferation of C4‐1 cells (derived from a carcinoma of the uterine cervix), whereas this ratio decreased in W1‐38 cells (normal lung fibroblasts). These results can be explained by differences between the transformed and non‐transformed cells in the cell‐density‐dependent transmembrane distribution and intracellular levels of cyclic nucleotides. In the serum‐deprived cultures, no profound effects were seen on the cell‐density‐dependent biokinetics of cAMP and cGMP. In the absence of serum, growth of C4‐1 cells was markedly retarded, whereas W1‐38 cells were unable to expand at all.