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Expression and chromosomal assignment of PTPH1 gene encoding a cytosolic protein tyrosine phosphatase homologous to cytoskeletal‐associated proteins
Author(s) -
Itoh Fumio,
Ikuta Shigeo,
Hinoda Yuji,
Arimura Yoshiaki,
Ohe Mutsumi,
Adachi Masaaki,
Ariyama Takeshi,
Inazawa Johji,
Imai Kohzoh,
Yachi Akira
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910550612
Subject(s) - homologous chromosome , protein tyrosine phosphatase , gene , cytosol , biology , cytoskeleton , genetics , microbiology and biotechnology , tyrosine , phosphatase , biochemistry , phosphorylation , enzyme , cell
We have investigated the mRNA expression of 2 human protein tyrosine phosphatases with sequence homology to cytoskeletal proteins, PTPHI and PTPMEG. Northern‐blot analysis of PTPHI using poly (A) + RNA from normal human colon tissue showed a low‐abundance message of 4.3 kb. Reverse‐transcriptase/polymerase‐chain reaction (RT‐PCR) was therefore used to detect it in a wide variety of cell lines including 9 colorectal, 5 gastric, 5 hepatic and 6 hematopoietic tumor cells. PTPHI mRNA was not detected only in Colo 320 cells over‐expressing c‐myc mRNA, among the colorectal cancer cell lines examined. When Colo 320 cells were incubated with 5 mM sodium butyrate for 5 days, PTPH I mRNA became detectable, concomitant with the marked decrease in the expression level of c‐myc mRNA. Moreover, the chromosomal localization of PTPHI gene was investigated by fluorescence in situ hybridization. Interestingly, PTPHI gene was mapped to 9q31 where the gene for Gorlin syndrome, a putative tumor suppressor gene, exists.