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Spontaneous development of a chromosomal translocation 5;14 in an epstein‐barr‐virus‐associated b‐cell lymphoma in a SCID mouse
Author(s) -
Glaser Ronald,
Theil Karl S.,
Bonneau Robert H.,
Sheridan John F.,
Vasquez Marco,
Allen Carl M.
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910550219
Subject(s) - chromosomal translocation , biology , clone (java method) , karyotype , antibody , virus , virology , lymphoma , epstein–barr virus , b cell , sendai virus , immunoglobulin heavy chain , microbiology and biotechnology , immunology , chromosome , genetics , gene
C.B‐17 SCID mice were inoculated with human peripheral blood leukocytes (PBLs) from normal Epstein‐Barr virus (EBV)‐seropositive and ‐seronegative donors. Confirmation of a functioning human immune response was demonstrated by the detection of human antibody after inoculation with rotavirus, tetanus toxoid, or EBV. One group of animals inoculated with PBLs from an EBV‐seropositive donor developed immunoblastic lymphomas approximately 9 weeks after transplantation. Confirmation of the species and sex of origin of the tumor cells was established using a spontaneous cell line prepared from the tumor. At passage I, the tumor‐cell line (AGTI) showed 15% of the metaphases with a translocation involving chromosomes 5 and 14. A lymphoblastoid cell line (AGLCL) established from the same PBLs from the same donor at the time of inoculation of the mice had a normal female karyotype. The AGLCL and a clone of AGTI cells were analyzed for rearrangement of immunoglobulin heavy chain (IgH) genes; both cell lines showed rearrangement of both IgH alleles. The results outlined in this report suggest that a spontaneous chromosomal translocation involving chromosome 14 occurred in normal PBLs in the SCID mouse.