Premium
Quantitation of HTLV‐I provirus among seropositive blood donors: Relation with antibody profile using synthetic peptides
Author(s) -
Matsumura Masayuki,
Kushida Shigeki,
Ami Yoshihiro,
Suga Tetsuya,
Uchida Kazuhiko,
Kameyama Tsuneo,
Terano Akira,
Inoue Yukiko,
Shiraki Hiroshi,
Okochi Kazuo,
Sato Hiroyuki,
Miwa Masanao
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910550209
Subject(s) - provirus , peripheral blood mononuclear cell , titer , virology , antibody , immunology , biology , medicine , in vitro , gene , biochemistry , genome
To study the pathophysiological state of HTLV‐1 carriers and the quantitative aspect of the risk of HTLV‐1 infection, we measured the absolute amounts of HTLV‐1 provirus in 39 seropositive blood donors. The amount of provirus varied from less than one to more than 10 4 molecules per 10 5 peripheral‐blood mononuclear cells (PBMC). The average amount of HTLV‐1 provirus among seropositive blood donors was estimated as 3.7 × 10 3 molecules per 10 5 PBMC. The titer of the antibody against only the synthetic peptide (aa 100 to 130) of the gag p19 region of the HTLV‐1 provirus showed a weak but significant correlation with the amount of the provirus.