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Characterization of four new cell lines derived from small‐cell gastrointestinal carcinoma
Author(s) -
Fujiwara Takato,
Motoyama Teiichi,
Ishihara Noriko,
Watanabe Hidenobu,
Kumanishi Toshiro,
Kato Kanefusa,
Ichinose Hiroshi,
Nagatsu Toshiharu
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910540617
Subject(s) - cell culture , small cell carcinoma , biology , large cell , carcinoma , cell , adenocarcinoma , cancer , small cell lung carcinoma , cancer cell , pathology , cancer research , microbiology and biotechnology , biochemistry , medicine , genetics
Four human small‐cell gastrointestinal carcinoma cell lines were established from tumor tissues of patients with esopha‐geal, gastric or rectal cancer, and were studied morphologically and biochemically in comparison with small‐cell lung carcinoma (SCLC) cell lines and common gastric cancer cell lines. Cells from all the small‐cell gastrointestinal carcinoma lines were as small as classic SCLC cells and had characteristic neurosecre‐tory granules. Cells from only one line grew as tightly packed spherical aggregates of floating cells, and those of the other 3 grew attached to substrate. Although high levels of creatine kinase brain isoenzyme (CK‐BB) were detected in all 4 cell lines, 2 of them showed low levels of aromatic L‐amino‐acid decarbox‐ylase and 3 had low levels of neuron‐specific enolase (NSE). None of the lines showed simultaneous elevation of enzymes. C‐ myc , N‐ myc , and L‐ myc were not amplified in any of the cell lines, but c‐myc mRNA was expressed in 2 lines. Our findings indicate that all small‐cell gastrointestinal carcinoma cells examined belong to the variant type which is used in the classification of SCLC. Furthermore, the ECC18 line, derived from esopha‐geal cancer, seemed to be of true endocrine cell origin, while the 3 other small‐cell gastrointestinal carcinoma lines seemed to arise via neoplastic neometaplasia from adenocarcinoma cells to endocrine cells.