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Induction of mouse tenascin expression by a human sarcomatoid wilms' tumor cell line growing in nude mice
Author(s) -
Talts Jan Fredrik,
Aufderheide Enno,
Sorokin Lydia,
Ocklind Göran,
Mattsson Ragnar,
Ekblom Peter
Publication year - 1993
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.2910540524
Subject(s) - tenascin , laminin , extracellular matrix , wilms' tumor , tenascin c , stromal cell , biology , pathology , nude mouse , cancer research , cell culture , fibronectin , microbiology and biotechnology , medicine , genetics
A new cell line from a sporadic Wilms' tumor was established and extensively characterized. In nude mice, the tumor cells rapidly formed tumors which, in histological characteristics and extracellular‐matrix (ECM) composition resembled sarcomatoid Wilms' tumor. The tumor cells produced B chains of laminin, but no A chain, and laminin was deposited into the ECM in a punctate pattern typical of sarcomatoid tumors. Strong expression of tenascin was detected within the stromal ECM of the tumors. Species‐specific antibodies reacting either with human or with mouse tenascin showed that tenascin was exclusively derived from mouse host cells. The human Wilms' cell line thus induced a strong stromal response with increased deposition of tenascin. The cell line may be useful for studying the behavior of sarcomatoid Wilms' tumor cells and for identifying factors that stimulate synthesis of tenascin.